Abstract:
The purpose of treatment of pulpal exposure is to preserve vitality, healthy and promote healing of exposed pulp tissue. Fluocinolone acetonide (FA) is a potent topical glucocorticoid used in treatment of skin disorders and oral lesions. It may have a potential to promote tissue healing. The aim of this study was therefore to investigate the effects of FA (0.1 to 10µM) on type 1 collagen synthesis and in vitro calcification in human dental pulp cells. The Western blot analysis was performed to examine the effect of FA on type 1 collagen synthesis at 5 days. The result showed that 1 and 10 µM of FA significantly stimulated the synthesis of collagen for about 2-fold (p>0.05). The result was confirmed by reverse transcription polymerase chain reaction (RT-PCR) which indicated that 1 µM FA could significantly induce the expression of type 1 collagen mRNAs for about 2.8 times (p>0.05). Long term cultures were done to examine the in vitro calcification. After 28 days, the result showed no difference between FA-treated groups and the controls. These results demonstrated that FA enhanced type 1 collagen synthesis but not in calcification process. The results suggested that FA may be the potential substance as a pulp capping material.
