The antioxidant test, Ames' test and micronuclei test of chemical extract from White Kwao Krua Pueraria mirifica, Red Kwao Krua Butea superba, Black Kwao Krua Mucuna collettii and Kudzu Pueraria lobata

Abstract

Antioxidant activity assay by DPPH scavenging method was submitted to Pueraria mirifica from 28 provinces, Butea superba from 23 provinces and Mucuna collettii from 4 provinces in comparison with Pueraria lobata from China. Our studies revealed that Mucuna collettii from Chiang Rai exhibited the strongest activity (IC₅₀= 55.53 ± 2.66 µg/m1) in their population, Butea superba from Loei exhibited the strongest activity in their population (IC₅₀ = 227.08±0.38 µg/ml), Pueraria minfica from Uthai 'Mani exhibited the strongest activity in their population (IC₅₀ = 2,470.38 ± 37.81 µg/m1), Pueraria lobata showed the weakest activity (IC₅₀= 2,482 ± 66.11µg/m1). The correlation study between five isoflavone contents in tubers analysed by HPLC and antioxidant activity, it was found that the antioxidant activity was directly correlated to daidzein content. The highest antioxidant activity plants in each group was analysed for the mutagenic and antimutagenicity by Ames' test and micronucleus test. The results revealed that Pueraria minfica, Butea superba, Mucuna collettii and Pueraria lobata exhibited no mutagenicity in TA98 and TA100 strains either in the absence and presence of the activation enzyme. Pueraria minfica, Butea superba, Mucuna collettii and Pueraria lobata exhibited antimutagenic activity in TA98 and TA100 strains in the absence and presence of the activation enzymes. The mutagenic activity of Pueraria mirifica, Butea superba, Mucuna collettii and Pueraria lobata were also examined in animals using the micronucleus test. The result revealed that Pueraria mirifica, Butea superba, Mucuna collettii and Pueraria lobata at highest dose (16 g/powder) had no mutagenic effect in the 24, 48 and 72 hour test period.