Food additive-drug interaction induced mutagens and possible prevention

Abstract

Twenty-seven drug preparations were treated with sodium nitrite and the interaction products were tested for mutagenicity in the Salmonella typhimurium test system (Ames test). The use of spot test method showed that eleven preparations gave positive results; then, they were tested using preincubation method of the plate incorporation, five preparations were found mutagenic. Being treated with nitrite, bromazepam, chlordiazepoxide and isoniazid were capable of causing both base-pair substitution (detected with strain TA 100) and frameshift (detected with strain TA 98) mutations. The nitrite treated cimetidine and ranitidine were capable of causing only base pair substitution mutations. Ivygourd fiber and bovine serum albumin were determined for inhibition of mutagen formation. They were able to prevent mutagen formation of drug-nitrite interaction.