Development of porous hydroxyapatite particles as carriers of proteins in polycaprolactone scaffold for bone tissue engineering

Abstract

This study aimed to develop the porous hydroxyapatite (HAp) particles as a controlled release carrier of proteins were embedded in polycaprolactone (PCL) to fabricate porous HAp-PCL scaffolds. Proteins (Ovalbum in, Gelatin type B, Bovine serum album in and Crude bone protein from pork bone) had been entrapped within the HAp particles. HAp particles were synthesized by coprecipitation technique from dicalcium phosphate dihydrate (CaHP042H20, DCPD ) and calcium carbonate(CaC03 ). Incorporation of proteins was accomplished during the coprecipitation of the two reactants. The porous HAp-PC L scaffolds were prepared by solvent casting/particulate leaching method of salt particle 400 pm at 30 w t%. The controlled release of proteins from HAp particles and the HAp-PCL scaffolds was influenced by ionic interaction between molecules of proteins and HAp and investigated by U V -Visible spectrophotometry. Profile release o f CBP was the greatest from Hap particle and HAp-PCL scaffold. The potential for use of protein-loaded HA p-PCL as bone scaffolds was assessed by mouse-calvaria derived pre-osteoblastic cells, MC3T3-E1. Cell attachment, cell proliferation, and alkaline phosphatate (ALP) activity on protein-HAp PC L scaffolds were not significant difference due to noeffect from proteins. For mineralization, the quantification of calcium deposition was observed the highest intensity on CBP/HAp-PCL scaffold. It can say that crude bone protein/HAp-PCL scaffold was suitable for bone tissue engineering.