hemical constituents and biological activity from the stem barks of Croton oblongifolius Roxb. from Amphoe Pranburi, Prachuabkhirikhan Province

Abstract

One clerodane, hardwickiic acid (1), abietane diterpenoid compound, abieta- 7,13-dien-3-one (2), and one cleistanthane diterpenoid compound, cleistantha-4, 13 (17), 15-triene-3-oic acid (3), were isolated from the stem barks of Croton oblongifolius Roxb., which was collected from Amphoe Pranburi, Prachuabkhirikhan Province. The structure of these compounds were established by spectroscopic data including IR, MS, ID and 2D NMR techniques (DEPT, COSY, NOESY, HMBC and HMQC) and chemical transformation. All of the isolated compounds and their dirivative, were subjected to biological activity test against a panel of six cell lines including HS 27 (fibroblast), KATO (gastric), BT 474 (breast), CHAGO (lung), SW 620 (colon) and HEP-G2 (hepatoma). It was showed that compound 2 exhibited cytotoxic activity against all cancer cell with %survival values less than doxorubicin test.