Antiproliferative effects of Pueraria mirifica pueraria lobata butea superba and Mucuna collettii on human mammary carcinoma MCF-7 and cervical carcinoma Hela

Abstract

The effects of Pueraria mirifica, Pueraria lobata, Butea superba and Mucuna collettii extracts were performed by incubating the MCF-7 (mammary carcinoma) and HeLa (cervical carcinoma) with the extracts at 0, 0.1, 1, 100, 1000 µg/ml for 4 days. The protein content of the cell culture was determined and calculated for the growth percentage compared with the control group. P. mirifica extract at low concentration (1 µg/ml) showed significant proliferative effect (p<0.05) and significant antiproliferative effects at high concentrations (1000 µg/ml) (p<0.01) on MCF-7 cells. In case of HeLa cells, P.mirifica showed significant antiproliferative effect (100 and 1000 µg/ml) (p<0.05) whereas P. lobata showed no effect. B. superba showed no proliferative effect but significant antiproliferative effect at high concentrations (10, 100 and 1000 µg/ml for MCF-7 and 1000 µg/ml for HeLa) (p<0.01). M. Collettii showed significant antiproliferative effect (p<0.01) at high concentrations (1000 µg/ml) on both cell lines. The synergistic analysis were performed by incubating the extracts at the low concentration (1 µg/ml for P. mirifica and P. lobata; 10 µg/ml for B. superba and M. collettii) with 10⁻¹¹ M estradiol, all of the extracts did not show the different effect on the growth of both cell lines compared with the cells treated with estradiol alone. The treatment of the extracts at the high concentration (1000 µg/ml ) with 10⁻¹¹ M estradiol, all of the extracts showed significant synergistic antiproliferative effect on both cell line compared with the cells treated with the extradiol alone.