Amperometric glucose sensor using hexaammineruthenium (III) chloride-nafion coated gold electrode for flow injection analysis

Abstract

Investigates new preparation procedures of amperometric glucose sensor. The enzyme glucose oxidase was immobilized on the surface of hexaamineruthenium (III) chloride-Nafion coated gold electrode by entrapment in electropolymerized pyrrole film. Ru(NH3)6Cl3, serve as electron mediator, was immobilized by the electrostatic interaction between [Ru(NH3)6]3+ and sulfonate groups of Nafion. The glucose determinations were performed using flow injection system. Glucose responses were determined by amperometry. With these glucose sensor, it was found that a detection limit was 5 mM and sensitivity was 1.47 nA/mM. The response time was 25-50 seconds. The operational stability of the glucose sensor was at least 12 times.