RAPD analysis for studying genetic diversity in five different breeds in gallopheasants and partridge

Abstract

Randomly Amplified Polymorphic DNA (RAPD) analysis was used to examıne genetic diversity in five different breeds in gallopheasants and partridge. Most of studying species are threatened birds of Thailand including Great Argus (Argusianus argus argus). Red Junglefowl (Gallus gallus spadhceous), Siamese Fireback (Lophura diardi), Green Peafowi (Pavo muticus imperator) anc Crested Wood Partridge (Rollulus roulroul) DNA templates in this study were extracted from bloodstains and feather pulp frorn wildlife research and breeding stations and private farms in vanous parts of Thailand. Screenıng from five out of a hundred primers (OPA 18, OPC-02, OPP-03, UBC-133 and UBC-135) shown highly polymorphic amplified products, For this study, RAPD analysis is possibly to analyzed. genetic diversity both in interspecies and intraspecies of gallopheasant Genetic distance in interspecies were ranging from 0.2297-04519 and genetic distance in intraspecies for Great Argus, Red Junglefowl, Siamese Fireback, Green Peafowl and Crested Wood Partridge were ranging from 0.0706-0.2599, 0.0889-0.2283, 0.0801-0.4500, 0.0710-0.5949 and 0.2376- 0.3990, respectively. Phylograms were constructed by using neighbor-joining, these unrooted trees can be described genetic relationships among interspecies and intraspecies. Genetic differences analysis by using pairwise exact test in all population are not significantly different in genetic distance (PS0.05) among interspecies and intraspecies. In this study was found candidate population specific bands among interspecies and intraspecies and its can be used to characterize in some different population such as Great Argus, Green Peafowl and Crested Wood Partridge.