Polypyrrole-coated poly(lactic acid) electrospun fibrous scaffold : effect of the coating layer on electrical conductivity and neutral cell growth

Abstract

Poly (lactic acid) (PLA) solution dissolved in chloroform was electro spun to produce nano-fibrous meshes as the fibrous scaffold. The average diameter of aligned and random of PLA fiber meshes were 1.744 ± 0.795 µm and 2.069 ± 0.577 µm respectively. Polypyrole (PPy) was coated on the PLA fiber meshes via admicellar polymerization of pyrrole (Py) monomer, carried out using 0.8 mM. (below CMC) aqueous DBSA solution at pH 4 (adjusted by using HCI) as surfactant. The average diameter of PPy-coated aligned and random PLA fiber meshes were increased. Furthermore, important physical properties of PPy-coated PLA fiber meshes was surface conductivity found that the conductivity at condition DBSA:Py monomer (1:8) PPy-coated PLA fiber meshes were increased in range 25-30 S/cm. In Biological characterizations found that cytocompatibility on PPy-coated PLA fiber meshes were examined using Neuro 2a as reference cell. Experimental results from MTT assay showed that PPy-coated PLA fiber meshes did not release substances toxicity and also indicated cell attachment on fiber meshes. In addition, PPy-coated aligned PLA fiber meshes were improve by Laminin coated on the electro spun fiber mesh found that increase cells interact with scaffolds. In electrical stimulation at 100 mV for 2 hours, Laminin coated on (PPy-coated aligned fiber meshes) is showed expression C-Fos gene of rat hippocampal neural stem cell increase compare to nonelectrical stimulation.